CS ROSETTA is a protocol which generates 3D models of proteins, using only the 13CA, 13CB, 13C', 15N, 1HA and 1HN NMR chemical shifts as input. Based on these parameters, CS ROSETTA uses a SPARTA-based selection procedure to select a set of fragments from a fragment-library (where the chemical shifts and the 3D structure of the fragments are known). The fragments are assembled using the ROSETTA protocol. The generated models are rescored based on the difference between the back-calculated chemical shifts of the generated models and the input chemical shifts. For more information see our online web portal manual and tutorial and/or consult the CS-Rosetta home page at: spin.niddk.nih.gov/bax/software/CSROSETTA/index.html.
Webserver user manual and tutorial
CS ROSETTA webserver
To use the CS ROSETTA webserver you must have registered for an account. If you do not have a account yet you can register here

The CS-Rosetta3 service is currently not in operation because of a hardware problem. Sorry for the inconvenience.

Setup your CS-Rosetta run
Give your run a name
What type of file do you submit?
Chemical Shift list
Number of models to generate
Exclude flexible parts?

Please exclude flexible parts manually.


Setup your rescoring method(s)
Chemical Shift Rescoring
DP Score
Noesy peaklist
N15 noesy peaklist
C13 aliphatic peaklist
C13 aromatic peaklist
C13 noesy peaklist
CC noesy peaklist

Advanced Options
Smoothing Rescoring

Example of CS ROSETTA webserver output
CS-ROSETTA webserver statistics
Server statistics generated on: 2017-06-28 14:21:50
Total number of active requests: 0
Number of requests running on the GRID: 0
Number of requests pending for GRID submission: 0
Total number of successfully served requests as of April 1st 2009: 4
Number of registered users: 81
References for use of the WeNMR GRID-enabled server
T.A. Wassenaar, M. van Dijk, N. Loureiro-Ferreira, G. van der Schot, S.J. de Vries, C. Schmitz, J. van der Zwan, R. Boelens, A. Giachetti, L. Ferella, A. Rosato, I. Bertini, T. Herrmann, H.R.A. Jonker, A. Bagaria, V. Jaravine, P. Guntert, H. Schwalbe, W.F. Vranken, J.F. Doreleijers, G. Vriend, G.W. Vuister, D. Franke, A. Kikhney, D.I. Svergun, R. Fogh, J. Ionides, E.D. Laue, C. Spronk, S. Jurka, M. Verlato, S. Badoer, S. Dal Pra, M. Mazzucato, E. Frizziero and A.M.J.J. Bonvin
"WeNMR: Structural Biology on the Grid."
J. Grid. Comp., 10, 743-767 (2012).
And add in addition the following text to the acknowledgment section:

"The WeNMR project (European FP7 e-Infrastructure grant, contract no. 261572, www.wenmr.eu), supported by the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands (via the Dutch BiG Grid project), Portugal, Spain, UK, South Africa, Taiwan and the Latin America GRID infrastructure via the Gisela project is acknowledged for the use of web portals, computing and storage facilities."